The differentiation antigen Ly-6E.1 is expressed in mouse metastatic tumor cell lines

We report the cloning of the mouse surface GPI-anchored Ly-6E.1 protein from a highly metastatic mouse adenocarcinoma cell line CSML-100 by differential display. The expression is specific for the metastatic cell line as the closely related, non-metastatic mouse adenocarcinoma cell line CSML-0 does...

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Veröffentlicht in:	FEBS letters 1997-02, Vol.403 (2), p.181-185
Hauptverfasser:	Cohn, Martin A, Kramerov, Dmitri, Hulgaard, Egil F, Lukanidin, Eugene M
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenocarcinoma - immunology Adenocarcinoma - pathology Adhesion Amino Acid Sequence Animals Antigens, Ly - genetics Antigens, Ly - immunology Antigens, Ly - metabolism base pair(s) Cell Differentiation - immunology Cloning, Molecular Differential display dl-dithiothreitol DNA, Complementary DTT glycosyl phoshatidyl inositol Glycosylphosphatidylinositols - metabolism GPI Ly-6E.1 Ly-6E.l mAb Metastasis Mice Molecular Sequence Data monoclonal antibody Neoplasm Metastasis - immunology pAb PAGE phenylmethylsulfonyl fluoride PMSF polyacrylamide gel electrophoresis polyclonal antibody reverse transcription-polymerase chain reaction RT-PCR SDS Sequence Homology, Amino Acid sodium dodecyl sulphate Tumor Cells, Cultured Tumor progression
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Zusammenfassung:	We report the cloning of the mouse surface GPI-anchored Ly-6E.1 protein from a highly metastatic mouse adenocarcinoma cell line CSML-100 by differential display. The expression is specific for the metastatic cell line as the closely related, non-metastatic mouse adenocarcinoma cell line CSML-0 does not express Ly-6E.1. Northern blot analysis reveals expression in a number of mouse tumour cell lines, exclusively metastatic ones. To date, active Ly-6A/E has only been described in lymphoid cells. The correlation between Ly-6E.1 expression, and the ability to metastasize, is discussed. © Federation of European Biochemical Societies.
ISSN:	0014-5793 1873-3468 1873-3468
DOI:	10.1016/S0014-5793(97)00050-1