Short‐ and long‐read metabarcoding of the eukaryotic rRNA operon: Evaluation of primers and comparison to shotgun metagenomics sequencing

High‐throughput sequencing‐based analysis of microbial diversity has evolved vastly over the last decade. Currently, the go‐to method for studying microbial eukaryotes is short‐read metabarcoding of variable regions of the 18S rRNA gene with

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Veröffentlicht in:	Molecular ecology resources 2022-08, Vol.22 (6), p.2304-2318
Hauptverfasser:	Latz, Meike A. C., Grujcic, Vesna, Brugel, Sonia, Lycken, Jenny, John, Uwe, Karlson, Bengt, Andersson, Agneta, Andersson, Anders F.
Format:	Artikel
Sprache:	eng
Schlagworte:	16S 18S Community DNA Primers Environmental monitoring Eukaryota eukaryote Eukaryotes genetics high throughput sequencing High-Throughput Nucleotide Sequencing marine plankton metabarcoding Metagenomics microbial eukaryotes Microorganisms operon PacBio long-read sequencing phylogeny primer design primer DNA Primers procedures Ribosomal RNA RNA 16S RNA 18S rRNA 18S rRNA 28S rRNA operon Taxonomy
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Beschreibung
Zusammenfassung:	High‐throughput sequencing‐based analysis of microbial diversity has evolved vastly over the last decade. Currently, the go‐to method for studying microbial eukaryotes is short‐read metabarcoding of variable regions of the 18S rRNA gene with
ISSN:	1755-098X 1755-0998 1755-0998
DOI:	10.1111/1755-0998.13623