Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua

The combination of propidium monoazide (PMA) and quantitative real-time PCR (qPCR) significantly overestimated the fraction of viable Listeria innocua as compared to plate counts and confocal fluorescence microscopy. Our data imply that PMA-qPCR must be used with caution as an analytical tool for th...

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Veröffentlicht in:	Journal of microbiological methods 2011-05, Vol.85 (2), p.164-169
Hauptverfasser:	Løvdal, Trond, Hovda, Maria Befring, Björkblom, Benny, Møller, Simon G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Azides - chemistry bacteria Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Colony Count, Microbial fluorescence microscopy Fundamental and applied biological sciences. Psychology Hot Temperature Listeria - chemistry Listeria - genetics Listeria - growth & development Listeria - isolation & purification Listeria innocua Microbial Viability Microbiology Microbiology (medical) Molecular Biology plate count Polymerase Chain Reaction - methods propidium Propidium - analogs & derivatives Propidium - chemistry Propidium monoazide quantitative polymerase chain reaction Quantitative real-time PCR Staining and Labeling - methods Viability
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Zusammenfassung:	The combination of propidium monoazide (PMA) and quantitative real-time PCR (qPCR) significantly overestimated the fraction of viable Listeria innocua as compared to plate counts and confocal fluorescence microscopy. Our data imply that PMA-qPCR must be used with caution as an analytical tool for the differentiation between viable and dead bacteria.
ISSN:	0167-7012 1872-8359 1872-8359
DOI:	10.1016/j.mimet.2011.01.027