Production of biomethane, biohydrogen, and volatile fatty acids from Nordic phytoplankton biomass grown in blended wastewater

[Display omitted] •Phytoplankton biomass was used as a feedstock for CH4, H2, and VFA production.•CH4 and H2 yield was higher after enzymatic hydrolysis than alkaline pretreatment.•Volatile fatty acid production was also enhanced by enzymatic hydrolysis.•Surfaces used for biofilm cultivation have an impact on biomass composition. Upgrading carbon-negative microalgal biomass to biofuels and value-added products presents a three-pronged solution for waste treatment, carbon capture, and economically viable bioenergy production. Acidogenesis and methanogenesis are versatile processes at the core of anaerobic digestion systems, facilitating the conversion of diverse biogenic substrates into energy and a wide range of biobased products. The present study was conducted to integrate acidogenesis and methanogenesis for coproduction of biohydrogen, biomethane, and volatile fatty acids from Nordic phytoplankton consortia. For this purpose, microalgal consortia was cultivated in a raceway pond equipped with high surface area structures. Harvested microalgal biomass was subjected to thermoalkaline (2% NaOH solution at 121 °C) and enzymatic (cellulase) pretreatments. The hydrolysates of the pretreated biomass were inoculated with thermally treated sludge for acidogenic fermentation and with mixture of untreated sludge and cow dung (1:1 v/v ratio) for anerobic digestion. The acidogenic process produced a significant amount of biohydrogen (maximum 164.8 mL bioH2/VSload) along with volatile fatty acids (maximum 7.9 g COD/L), while methanogenesis resulted in biomethane production of maximum 210.7 mL bioCH4/VSload, accompanied by an ammonium recovery of 1278 mg NH4+/L. These maximum yields were all achieved by enzymatic pretreatment of the biomass fraction harvested from high surface area brush head structures inserted in the raceway pond. These results have important implications for designing phytoplankton cultivation systems and upstream pathways to optimize energy production from carbon-negative phytoplankton biomass.