Carbon and gold electrodes as electrochemical transducers for DNA hybridisation sensors

Genosensor technology relying on the use of carbon and gold electrodes is reviewed. The key steps of each analytical procedure, namely DNA-probe immobilisation, hybridisation, labelling and electrochemical investigation of the surface, are discussed in detail with separate sections devoted to label-...

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Veröffentlicht in:	Biosensors & bioelectronics 2004-01, Vol.19 (6), p.515-530
Hauptverfasser:	Lucarelli, Fausto, Marrazza, Giovanna, Turner, Anthony P.F, Mascini, Marco
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensors Biotechnology Carbon Carbon electrodes Coated Materials, Biocompatible - chemical synthesis DNA - analysis DNA - chemistry DNA - genetics DNA chips DNA hybridisation DNA Probes - chemical synthesis DNA sensors Electrochemical DNA biosensors Electrochemistry - instrumentation Electrochemistry - methods Electrodes Equipment Design Fundamental and applied biological sciences. Psychology Genosensors Gold Gold electrodes Humans In Situ Hybridization - instrumentation In Situ Hybridization - methods Methods. Procedures. Technologies PCR Polymerase Chain Reaction - instrumentation Polymerase Chain Reaction - methods TECHNOLOGY TEKNIKVETENSKAP Transducers Various methods and equipments
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Beschreibung
Zusammenfassung:	Genosensor technology relying on the use of carbon and gold electrodes is reviewed. The key steps of each analytical procedure, namely DNA-probe immobilisation, hybridisation, labelling and electrochemical investigation of the surface, are discussed in detail with separate sections devoted to label-free and newly emerging magnetic assays. Special emphasis has been given to protocols that have been used with real DNA samples.
ISSN:	0956-5663 1873-4235 1873-4235
DOI:	10.1016/S0956-5663(03)00256-2