The Cryotop vitrification system is competent for the simultaneous cryopreservation of large numbers of pig in vitro‐produced blastocysts

This study aimed to compare the effectiveness, in terms of viability and quality post‐warming, when vitrifying in vitro‐produced (IVP) pig blastocysts with either a modified Cryotop (n = 161; 20 blastocysts/device) or the conventional Superfine Open Pulled Straw (SOPS; n=160; 5‐6 blastocysts/device...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Reproduction in domestic animals 2024-10, Vol.59 (S3), p.e14600-n/a
Hauptverfasser:	González‐Plaza, A., Garcia‐Canovas, M., Parrilla, I., Rodriguez‐Martinez, H., Gil, M. A., Martinez, E. A., Cuello, C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apoptosis blastocyst Blastocyst - physiology Blastocysts Cell culture Cell Survival Cryopreservation Cryopreservation - methods Cryopreservation - veterinary Cryotop Effectiveness Embryo Culture Techniques - methods Embryo Culture Techniques - veterinary Embryo transfer Embryo Transfer - veterinary Embryos Female Fertilization in Vitro - veterinary in vitro‐produced blastocysts Parameters porcine SOPS Swine Ultrafines Viability Vitrification
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	This study aimed to compare the effectiveness, in terms of viability and quality post‐warming, when vitrifying in vitro‐produced (IVP) pig blastocysts with either a modified Cryotop (n = 161; 20 blastocysts/device) or the conventional Superfine Open Pulled Straw (SOPS; n=160; 5‐6 blastocysts/device systems. Blastocyst viability, morphology, and apoptosis parameters were evaluated after 24 h of in vitro culture. The Cryotop system yields better results in terms of higher embryo viability and total cell numbers (p
ISSN:	0936-6768 1439-0531 1439-0531
DOI:	10.1111/rda.14600