Resveratrol reduces oxidative stress and cell death and increases mitochondrial antioxidants and XIAP in PC6.3-cells

▶ RSV reduces oxidative stress and enhances cell survival in neuron-like. ▶ PC6.3 cells. ▶ RSV increases the levels of antioxidants, SOD2 and TRX2, in neuron-like. ▶ PC6.3 cells. ▶ RSV increases the levels of XIAP in neuron-like PC6.3 cells. ▶ RSV activates NFκB signaling in neuron-like PC6.3 cells. Resveratrol, a polyphenol derived e.g. from red grapes, has been shown to mediate several positive biological actions such as protection of cells against oxidative stress. It can also influence cell signaling, but the mechanisms behind its antioxidant properties are largely unknown. Here we show that RSV reduces oxidative stress and enhances cell survival in PC6.3 cells depending on the concentration. In these cells, RSV increased the levels of antioxidants, SOD2 and TRX2, and of X chromosome-linked inhibitor of apoptosis protein. RSV also activated NFκB signaling as shown using luciferase reporter constructs. These findings show that RSV regulates oxidative stress and mitochondrial antioxidants in neuronal cells. This may contribute to cell protection in various brain disorders.