Label-free detection of interleukin-6 using electrolyte gated organic field effect transistors

Cytokines are small proteins that play fundamental roles in inflammatory processes in the human body. In particular, interleukin (IL)-6 is a multifunctional cytokine, whose increased levels are associated with infection, cancer, and inflammation. The quantification of IL-6 is therefore of primary im...

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Veröffentlicht in:Biointerphases 2017-12, Vol.12 (5), p.05F401-05F401
Hauptverfasser: Diacci, Chiara, Berto, Marcello, Di Lauro, Michele, Bianchini, Elena, Pinti, Marcello, Simon, Daniel T, Biscarini, Fabio, Bortolotti, Carlo A
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Sprache:eng
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Zusammenfassung:Cytokines are small proteins that play fundamental roles in inflammatory processes in the human body. In particular, interleukin (IL)-6 is a multifunctional cytokine, whose increased levels are associated with infection, cancer, and inflammation. The quantification of IL-6 is therefore of primary importance in early stages of inflammation and in chronic diseases, but standard techniques are expensive, time-consuming, and usually rely on fluorescent or radioactive labels. Organic electronic devices and, in particular, organic field-effect transistors (OFETs) have been proposed in the recent years as novel platforms for label-free protein detection, exploiting as sensing unit surface-immobilized antibodies or aptamers. Here, the authors report two electrolyte-gated OFETs biosensors for IL-6 detection, featuring monoclonal antibodies and peptide aptamers adsorbed at the gate. Both strategies yield biosensors that can work on a wide range of IL-6 concentrations and exhibit a remarkable limit of detection of 1 pM. Eventually, electrolyte gated OFETs responses have been used to extract and compare the binding thermodynamics between the sensing moiety, immobilized at the gate electrode, and IL-6.
ISSN:1934-8630
1559-4106
1559-4106
DOI:10.1116/1.4997760