Production of therapeutic glycoproteins in glycoengineered plant: old farm for new crops

Plant-based expression systems have emerged as promising avenues for the production of recombinant N-linked glycoproteins. This review offers insights into the evolution and progress of plant glycoengineering. It delves into the distinctive features of plant-derived N-glycans, the diverse range of plant hosts employed for glycoprotein synthesis, and the advancements in glycoengineering strategies aimed at generating glycoproteins with N-glycan structures akin to those produced in mammalian cell lines. Furthermore, alternative strategies for augmenting glycoengineering efforts and the current spectrum of applications for plant-produced N-glycan recombinant proteins are examined, underscoring their potential significance in biopharmaceutical manufacturing. •Plant-based expression systems are promising for producing recombinant N-linked glycoproteins.•Review explores plant glycoengineering history and recent advances in innovative vectors.•Hosts like Nicotiana benthamiana, Nicotiana tabacum BY-2 cells, are investigated.•Significant progress in optimizing glycoengineering strategies for mammalian-like glycosylation.•Review highlights biopharmaceutical manufacturing using glycoengineered plants.