The knowns and unknowns of callose biosynthesis in terrestrial plants

Callose, a linear (1,3)-β-glucan, is an indispensable carbohydrate polymer required for plant growth and development. Advances in biochemical, genetic, and genomic tools, along with specific antibodies, have significantly enhanced our understanding of callose biosynthesis. As additional components of the callose synthase machinery emerge, the elucidation of molecular biosynthetic mechanisms is expected to follow. Short-term objectives involve defining the stoichiometry and turnover rates of callose synthase subunits. Long-term goals include generating recombinant callose synthases to elucidate their biochemical properties and molecular mechanisms, potentially culminating in the determination of callose synthase three-dimensional structure. This review delves into the structures and intricate molecular processes underlying callose biosynthesis, emphasizing regulatory elements and assembly mechanisms. [Display omitted] •Highlighting the critical role of callose biosynthesis in plant growth and defense.•Reviewing the composition of callose synthase complexes and the current understanding of callose assembly mechanisms.•Exploring genes encoding callose synthase and regulatory elements across various plant developmental stages.