Detection and Analysis of Low-Abundance Cell-Surface Biomarkers Using Enzymatic Amplification in Microfluidic Droplets

Finding the few: Cell-surface proteins are useful disease biomarkers, but current high-throughput methods are limited to detecting cells expressing more than several hundred proteins. Enzymatic amplification in microfluidic droplets (see picture) is a high-throughput method for detection and analysi...

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Veröffentlicht in:	Angewandte Chemie (International ed.) 2009-01, Vol.48 (14), p.2518-2521
Hauptverfasser:	Joensson, Haakan N, Samuels, Michael L, Brouzes, Eric R, Medkova, Martina, Uhlén, Mathias, Link, Darren R, Andersson-Svahn, Helene
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens, CD19 - analysis Biomarkers - analysis Cell Line devices droplets encapsulation enzymatic amplification Fluorescence gene high-throughput screening Humans Membrane Proteins - analysis microdroplets Microfluidic Analytical Techniques - methods microfluidics molecules picoliter droplets polymerase-chain-reaction protein expression proteins Receptors, CCR5 - analysis single cells therapy U937 Cells
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Beschreibung
Zusammenfassung:	Finding the few: Cell-surface proteins are useful disease biomarkers, but current high-throughput methods are limited to detecting cells expressing more than several hundred proteins. Enzymatic amplification in microfluidic droplets (see picture) is a high-throughput method for detection and analysis of cell-surface biomarkers expressed at very low levels on individual human cells. Droplet optical labels allow concurrent analysis of several samples.
ISSN:	1433-7851 1521-3773 1521-3773
DOI:	10.1002/anie.200804326