Insertion sequence profiling of UK Mycoplasma bovis field isolates
The presence and distribution of insertion sequences IS Mbov2 and IS Mbov3 within Mycoplasma bovis were investigated. Analysis was carried out by Southern blotting using specific probes of 221 bp and 185 bp, to detect IS Mbov2 and IS Mbov3, respectively, amplified from the homologous sequences IS Mm...
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Veröffentlicht in: | Veterinary microbiology 2005-05, Vol.107 (3), p.301-306 |
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Sprache: | eng |
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Zusammenfassung: | The presence and distribution of insertion sequences IS
Mbov2 and IS
Mbov3 within
Mycoplasma bovis were investigated. Analysis was carried out by Southern blotting using specific probes of 221
bp and 185
bp, to detect IS
Mbov2 and IS
Mbov3, respectively, amplified from the homologous sequences IS
Mmy1 and IS1634 within
Mycoplasma mycoides subspecies
mycoides small colony type. We present data obtained from 49 field isolates of
M. bovis, originating from pneumonic lungs, collected within the United Kingdom between 1996 and 2002. Hybridisation profiles show considerable variation between strains. IS
Mbov2 sequences are present between 2 and 17 copies while there are between 3 and 14 copies of the IS1634 homologue IS
Mbov3. These data also provide support for previous analysis by random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP). |
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ISSN: | 0378-1135 1873-2542 1873-2542 |
DOI: | 10.1016/j.vetmic.2005.02.003 |