A Water-Soluble Fluorescent Probe for the Selective Sensing of Ag+ and its Application in Imaging of Living Cells and Nematodes

In this study, an imidazole-coumarin based fluorescent probe was developed for the selective and sensitive detection of Ag + in aqueous solution. Using a combination of Job plot, NMR titrations, and DFT calculations, the binding properties between Ag + and the probe were deeply investigated, and the results revealed a 1:1 binding stoichiometry between the probe and Ag + with a binding constant of 1.02 × 10 6 M −1 . The detection limit was found to be 150 nM, which satisfies the requirement for the quantitative detection of Ag + in real water samples. Moreover, the new probe, Ic , was successfully applied to sense Ag + in HeLa and HepG2 cells as well as in C. elegans , indicating that it could be a useful tool for the environmental monitoring of Ag + pollution. These results demonstrated that Ic could serve as a high-efficiency and low-cost fluorescent probe for tracking Ag + in an aquatic environment and biological organisms.