Detection of MspI RFLP in human THY1 gene by the polymerase chain reaction

Detection of MspI RFLP in human THY1 gene by the polymerase chain reaction

THY1 gene encodes a cell surface glycoprotein predominantly expressed in brain and peripheral nerves. Human THY1 gene region on chromosome 11q23 has been implicated in susceptibility to type 1 diabetes (Wong et al. , 1991). Two primers derived from the sequences flanking the polymorphic MspI site in...

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Veröffentlicht in:Japanese journal of human genetics 1995-09, Vol.40 (3), p.279-280
Hauptverfasser: Fu, Jian, Ikegami, Hiroshi, Yamato, Eiji, Kawaguchi, Yoshihiko, Takekawa, Kiyoshi, Fujisawa, Tomomi, Nakagawa, Yusuke, Hamada, Yoichi, Ueda, Hironori, Shen, Gong Qing, Ogihara, Toshio
Format: Artikel
Sprache:eng
Schlagworte:
Base Sequence
Biomedical and Life Sciences
Biomedicine
Deoxyribonuclease HpaII
Gene Expression
Gene Function
Gene Therapy
Human Genetics
Humans
Molecular Medicine
Molecular Sequence Data
Polymerase Chain Reaction - methods
Polymorphism, Restriction Fragment Length
RFLP Report
Thy-1 Antigens - genetics
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Zusammenfassung:THY1 gene encodes a cell surface glycoprotein predominantly expressed in brain and peripheral nerves. Human THY1 gene region on chromosome 11q23 has been implicated in susceptibility to type 1 diabetes (Wong et al. , 1991). Two primers derived from the sequences flanking the polymorphic MspI site in intron 2 of the human THY1 gene (Gatti et al. , 1988) were selected for RCP to amplify a 566 bp fragment that spans the MspI polymorphism. Polymorphism was detected by MspI digestion of the PCR product.
ISSN:1434-5161
0021-5074
0916-8478
1435-232X
DOI:10.1007/BF01876187