Studying the Human Immunome: The Complexity of Comprehensive Leukocyte Immunophenotyping

A comprehensive study of the cellular components of the immune system requires both deep and broad immunophenotyping of numerous cell populations in an efficient and practical manner. In this chapter, we describe the technical aspects of studying the human immunome using high-dimensional (15 color)...

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Veröffentlicht in:	Current topics in microbiology and immunology 2014-01, Vol.377, p.23-60
Hauptverfasser:	Biancotto, Angélique, McCoy, J. Philip
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibody Cocktail BIOLOGY, LIFE SCIENCES Biomarkers - metabolism Cell Tube Chronic Lymphocytic Leukemia Chronic Lymphocytic Leukemia Patient Flow Cytometry - instrumentation Flow Cytometry - methods Fluorescent Dyes - chemistry Humans Immunology Immunophenotyping - instrumentation Immunophenotyping - methods Leukocyte Subset Leukocytes - chemistry Leukocytes - cytology Leukocytes - metabolism Oncology
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Beschreibung
Zusammenfassung:	A comprehensive study of the cellular components of the immune system requires both deep and broad immunophenotyping of numerous cell populations in an efficient and practical manner. In this chapter, we describe the technical aspects of studying the human immunome using high-dimensional (15 color) fluorescence-based immunophenotyping. We focus on the technical aspects of polychromatic flow cytometry and the initial stages in developing a panel for comprehensive leukocyte immunophenotyping (CLIP). We also briefly discuss how this panel is being used and the challenges of encyclopedic analysis of these rich data sets.
ISSN:	0070-217X 2196-9965
DOI:	10.1007/82_2013_336