Pancreatic Differentiation from Murine Embryonic Stem Cells
Pluripotent stem cells are considered as a cell source for replacement therapies for pancreatic beta cells and other organs. We identified tetrabenazine (TBZ), vesicular monoamine transporter 2 (VMAT2) inhibitor as a promoter of late-stage differentiation of Pdx1-positive pancreatic progenitor cells...
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Veröffentlicht in: | Embryonic Stem Cell Protocols 2016-01, Vol.1341, p.417-423 |
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Sprache: | eng |
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Zusammenfassung: | Pluripotent stem cells are considered as a cell source for replacement therapies for pancreatic beta cells and other organs.
We identified tetrabenazine (TBZ), vesicular monoamine transporter 2 (VMAT2) inhibitor as a promoter of late-stage differentiation of Pdx1-positive pancreatic progenitor cells into Ngn3-positive endocrine progenitor cells. A cell-permeable cAMP analog, dBu-cAMP promotes beta cell maturation in late stage of differentiation. The induced beta cells can secrete insulin in a glucose-dependent manner.
Our protocol consists of a three -step differentiation process. ES cell recapitulate embryonic developmental processes in vitro. Therefore, the ES cell differentiation system is a useful model for the understanding of molecular mechanism of beta-cell differentiation and are useful for application for future regenerative medicine. |
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ISSN: | 1064-3745 1940-6029 |
DOI: | 10.1007/7651_2015_217 |