Synthesis of camphor oxazole from isolates of sumatran camphor (Dryobalanops aromatica) and its antioxidant activity using the free radical DPPH method

Dryobalanops aromatica or also known as Sumatran camphor is an endemic flora that can be found on the islands of Sumatra and Kalimantan. Dryobalanops aromatica is a producer of borneol/camphor compounds in oil or crystal form. In this study, the crystals were isolated from Dryobalanops aromatica oil and then oxidized to camphor. The synthesis was continued by reacting camphor with semicarbazide and ethyl-2-chloro acetoacetate to form oxazole camphor with sodium acetate catalyst. The formation of D. aromatica crystals, camphor crystal, and camphor oxazole was then characterized using thin-layer chromatography (TLC), FT-IR, UV-Vis, and GC-MS/LC-MS. D. aromatica crystal, camphor crystal, and camphor oxazole were successfully synthesized with a mass of 0.6211, 0.1564, and 0.5535 grams, respectively. Antioxidant activity was measured using the DPPH free radical method. Camphor oxazole compound has a very strong antioxidant activity with an IC50 value of 11.03 ppm which is a very strong antioxidant activity. Meanwhile, Dryobalanops aromatica oil has an IC50 value of 4293 ppm, D. aromatica crystal has a value above 10000 while camphor has no antioxidant activity.