Development of method for in vitro evaluaton of radiosensitivity of human brain glial tumors

The aim of the study was to develop a method for evaluation of individual sensitivity of human brain glial tumors using live tumor slices with maximum preservation of the structural organization. Fragments of tumors removed during surgery were used for the study. The samples were stored in sterile saline solution with 5% glucose, cooled to 4 ° C. From the obtained fragments of tumors, slices of human brain tumor tissues with thickness of 3 to 5 mm were prepared. Five slices were prepared from each individual tumor sample, one slice was fixed immediately and used for histological analysis of the control, the second slice was used as control and incubated for 24 hours, the remaining three slices were used for gamma-irradiation with absorbed doses of 5, 10 and 15 Gy. After irradiation, the slices were transferred to fresh saline with 5% glucose, cooled to 4°C, and incubated for 24 hours at 4°C, then fixed in 10% formalin, followed by histological analysis of the degree of tumor tissue necrosis. The radiosensitivity of glial tumor tissues was assessed using a specially developed scale. Studies were conducted on various types of glial tumors in 200 patients and the presence of resistant forms of tumors in individual patients was shown. The data obtained suggest that the developed method can be successfully used for individual prediction of the effectiveness of radiation therapy in patients with glial tumors.