Rapid test of bioethanol raw material: The Weende-Chesson-Datta method modification to fiber cellulose

Lignocellulose in plant residues from land and aquatic environments is a very abundant source of organic matter especially from the agricultural sector for bioethanol raw materials. Cellulose fraction in fibers is very important to know and now there is no rapid analysis approach to estimating the amount of glucose for feasibility of bioethanol raw materials (BRM) from environmental resources. How to obtain cellulose content valuation that is easily applied and fast to all type of plants. The aim of research is to compile a fast way of cellulose analysis from resources of all environmental sectors. Research uses descriptive to analysis method implementation and the experimental method with design of one shot-study by measuring the quantity of glucose. The independent variables were hydrolyzed agent; hydrochloric acid and sulphuric acid, Varies concentration of sulphuric acid; 0.5 M and 72%, a long time of soaking; 1, 2, 3, 4 hours in sulphuric acid 72%, initial hydrolysis by concentration of sulphuric acid; 0.5 M and 72%. The glucose yield was determined by physical method. The modification Weende-Chesson method without carrying out the ash fraction and was continued with the Datta method. Dry material containing minerals is hydrolyzed with sulfuric acid 0.5M at 100°C in reflux, then two hours soaking stage of sulfuric acid 72%, and dilution to 0.5M concentration. Technical modification provided glucose (246.889 ± 4.345) mole/Kg from residual content in Ananas cosmosus var. Gandusari leaf and is higher than var. Cayene. Proposing method of cellulose valuation from plants is technical modification of the method for opening lignin and hydrolyzing hemicellulose and cellulose.