Resistance level in the middle generation of mutant chili genotypes as a result of gamma rays irradiation against Pepper yellow leaf curl Virus (PepYLCV)

A resistant variety is needed to overcome a pest or disease infection. The research purposes were to obtain the selected M3 chili that was not only resistant to PepYLCV but also had a high yield and good quality. The research was conducted at Indonesian Vegetables Research Institute (IVEGRI), Lembang (1,250 m sal) from May to December 2017. The augmented design with three replications for each control genotype was used in the research. The infected chili plants by PepYLCV and eggplant were placed at certain points around the field as a natural inoculum and plant host for insect vector white flies to ensure no escape condition. Resistance selection was conducted according to the standardized disease scoring value (0 – 4) in the field at a certain period of time, then calculated disease incident intensity and AUDPC value parameters. IPBC-12 variety was used as a resistant control. Selection of yield quantity-quality according to the standardized multicharacter with referring to consumer’s preference i.e. AUDPC value≤resistant genotype (IPBC-12), productivity≥1,0 kg/plant, fruit length (10-12 cm), fruit diameter (1.0-1.5 cm), capsaicin content≥300 ppm, and vitamin C content≥100 ppm. The result showed that the selected M3 lines were 17 or selection intensity around 26.56%. Relatively the tested M3 lines still had low uniformity on some morphologies. There was a transgressive segregation phenomenon that occurred on 21 M3 plant individuals. Nevertheless, the selected M3 genotypes had to be continually evaluated to obtain a new resistant mutant variety to PepYLCV.