Optimization of lipase activity from Muricauda aquimarina by pH and temperature conditions

Oil spill in marine waters is considered very harmful and polluting. The possibility of oil-degrading bacteria living in polluted waters is very high. Lipase is an enzyme that can break down triglycerides in oil into free fatty acids and glycerol. This study aims to optimize the lipase activity from Muricauda aquimarina by modifying pH and temperature. A confirmation lipase test was carried out using a medium containing olive oil and 0.1% rhodamine B dye on an agar plate and confirmed by orange fluorescence color under UV light. This study used OFAT (One Factor at A Time) condition. At the beginning of the pH optimization, the temperature was set to 37oC, and the variable of pH ranged from 4-8. The results showed that the highest enzyme activity was at 37oC and pH 6, with an activity value of 6.8 U/mL. Optimization of the incubation temperature ranged from 30-50oC, with the final result of optimization by 35oC pH 6 with an activity value of 9.11 U/mL and protein concentration 0.973 mg/mL giving the specific activity at 5.88 U/mg.