The lipid extract of Sargassum polycystum found in Lombok coast Indonesia acts against free radicals and diabetes mellitus

Indonesia is a country with a high biodiversity of algae, especially brown algae. Here, we studied the bioactivity of the lipid compounds in Indonesian brownalgae, Sargassum polycystum. Dried and powdered brown algae were extracted with chloroform:methanol:water. The lipid extract was collected and the solvent was evaporated. The characteristic of the compounds was then analysed by Thin Layer Chromatography with Silica Gel 60F254 as the stationary phase and petroleum ether:dietylether:acetic acid as the mobile phase. Upon elution, the lipid compounds were then detected with sulfur acid reagent. The anti-oxidant activity of the extract was then analysed by DPPH method, and the anti-diabetic effect of the compound was then analysed in the alloxan-induced diabetic rats. TLC analyses showed that the majorcompound in theSargassum polycystum lipid extract wasfattyacids. The lipid extractalsoshown anti-oxidantactivity in DPPHmethod, with IC50of460.5 µg/ml. Moreoverthe extract alsoloweredfastingbloodglucose level in alloxan-inducedrats. This study showsthatthebrownalgaelipid extract from LombokIndonesia coast, has anti-diabeticactivitythroughthe reductionofoxidativestress.