Acute toxicity test of ethanolic extract and n-hexane fraction of tiger milk (Lignosus rhinocerus sp.) against larva of artemia Salina L. using the brine shrimp lethality test (BSLT)

Tiger milk mushroom (Lignosus rhinocerus sp.) contains secondary metabolites, namely flavonoids and alkaloids which at certain levels could be toxic and have antimicrobial and anti-inflammatory activities. This study aims to determine the cytotoxic activity of ethanol extract and n-hexane fraction of tiger milk mushroom (Lignosus rhinocerus sp.) using the BSLT (Brine Shrimp Lethality Test) method. The characteristics of the ethanolic extract are greenish brown and the n-hexane fraction are brown with a special scent. This research was conducted using 48 hours of Artemia salina Leach larvae. The LC50 value was calculated using probit analysis. The results showed that the ethanol extract and n-hexane fraction of tiger milk mushroom (Lignosus rhinocerus sp.) had cytotoxic activity against Artemia salina Leach. with LC50 values of 446.68 ppm and 812.83 ppm.