Extraction of phenolic compounds from strobilanthes crispus with continuous ultrasound assisted extraction as antihypercholesterol drug preparation

Ultrasound Assisted Extraction (UAE) is a green extraction method which is one solution to increase yield and speed up extraction time. Research using conventional methods such as soxhlet, maceration, percolation and reflux has been carried out and has several weaknesses, including low yield, low selectivity and the use of large volumes of solvent, causing safety and environmental problems. The method used in this research is the Continuous Ultrasound Assisted Extraction (CUAE) method, which is one of the green extraction methods for extracting various types of compounds. The purpose of this study was to extract the leaves of Strobilanthes Crispus to obtain the total content of phenolic compounds and compounds that have antihypercholesterol activity. Extraction was carried out using various variables, namely extraction time and extraction temperature. The operating conditions used in this study were sample size 0.585 mm, cellulase enzyme concentration 30 mg/g, 50% ethanol concentration and the use of a sonicator with a frequency of 53 kHz and temperatures of 30 °C, 40 °C, 50 °C and 60 °C. The highest Total Phenolic Content (TPC) obtained by quantitative test using UV-Vis Spectrophotometer in this study was at a temperature of 50 °C at 2.864 mg GAE/g sample for 10 minutes of extraction. This shows that by using the Continuous Ultrasound Assisted Extraction (CUAE) method, it can speed up the extraction process with optimal results. Gas Chromatography-Mass Spectrometry (GC-MS) was used as a quantitative test of compounds having antihypercholesterol activity. The test results show that the extraction of Strobilanthes Crispus leaves using the Continuous Ultrasound Assisted Extraction (CUAE) method can produce several compounds that have activity that can be used as antihypercholesterol drugs, including octadecanoic acid and hexadesenoic acid.