Fractionation of ethanol extract of sesewanua leaves (Clerodendrum fragrans wild.) in n-hexane : Ethyl acetate and their antioxidant activity

Sesewanua (Clerodendrum fragrans Wild.) is a plant that is empirically used as an antitumor, anti-inflammatory, antipyretic, anti-breast cancer, and antioxidant. Sesewanua leaves contain flavonoid compounds, alkaloids, tannins, and saponins. This study aimed to identify the potential antioxidant activity of Sesewanua leaf ethanol extract (SWE) using 11 variations of solvent ratio of n-hexane: ethyl acetate. The method used was maceration with 96% ethanol solvent then a screening test is carried out followed by stain separation using TLC and fractionation using Column Chromatography with 11 ratios (10:0, 9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8, 1:9 and 0:10). The obtained fractions were continued to the antioxidant activity test using the DPPH method. The Retention factor (Rf) values of n-hexane: ethyl acetate fractions of SWE were N1: 0.375, N2: 0.475, N3: 0.525, N4: 0.6, and N5: 0 .75. Fraction 1 (10:0, 9:1, 8:2, 7:3, 6:4), fraction 2 (5:5, 4:6, 3:7), and fraction 3 (2:8, 1:9, 0:10) have IC50 value of 2.5603 µg/mL, 5.9382 µg/mL, and 2.7519 µg/mL, respectively. The n−hexane: ethyl acetate fractions of SWE contain secondary metabolites with very strong antioxidant activity.