Synthesis and characterization of tea tree oil using DLS and its nanoformulation to improve the efficacy against gram negative clinical pathogens

The major aim of this study is the characterization of tea tree oil using DLS and to improve its efficacy against gram negative bacteria. Tea tree oil nanoemulsion preparation was done by the standard protocol. Tea tree oil nanoemulsion (N=21) and control antibiotics (Gentamicin) and zone of inhibition was observed for the well and disc diffusion as per the procedure. Characterization of tea tree oil nanoemulsion using DLS and SEM for droplet size and particle size distribution. Independent sample T test was done which revealed that nano formulated tea tree oil showed comparatively good results against E.coli with respect to the control antibiotics. The zone of inhibition in well diffusion was found to be 15 ±2mm for TT oil nanoemulsion and 15mm for essential tea tree oil respectively (P>0.005). Average particle size distribution was achieved at 81 nm and with average particle size of tea tree oil nanoemulsion was 169 nm. Scanning electron microscopic image of tea tree oil nanoemulsion with average droplet size of 92±2nm. Nano formulated tea tree oil showed better results for droplet size and particle size and good zone of inhibition.