Synthesis and in vitro cytotoxic activity of compounds (E)-1-(3-bromophenyl)-3-(4-chlorophenyl)prop-2-en-1-on using breast cell line (MCF-7)

The synthesis compound (E)-1-(3-bromophenyl)-3-(4-chlorophenyl)prop-2-en-1 on as target compound and anticancer against MCF-7 breast cancer cells had been performed. The synthesis was performed by Claisen-Schmidt condensation by using 3’-bromoasetofenon as acetophenone and 4-chlorobenzaldehyde as benzaldehyde. The synthesis was carried out in alkaline condition (KOH) by microwave irradiation for 4 minutes. The structures of products were identified by IR, MS, 1H-NMR spectrometers. The method used for cytotoxicity was the addition of prestoblueTM reagent and absorbance measurement. The result showed that the chalcone derivatives was yielded in 74,34 % as yellow solid. Anticancer assays showed that chalcone had moderate activity against the breast cancer cell line (MCF-7), based on the IC50 values 29.27 µg/mL.