Spectrophotometric determination of mesalazine in pure and pharmaceutical preparations by chelation method

an accurate, simple, and precise spectrophotometric method is presented for the determination of Mesalazine based on formed chelating complex with Fe(III) . The intensity of complex measured at 508 nm after optimization of the experimental parameters. Beer's law was obeyed to the proposed method and it was valid within a concentration range of 2.5–22.5 μg/mL and the linear regression was R2=0.9980. Detection limit of quantitation was 0.0071 μg/mL, and the molar absorptivity coefficient 2.80x104 L.mol−1cm−1. Sandal's sensitivity was 0.0054 μg.cm−2 There is no interference from excipients found in the tablet. the current approach was effectively used to the determination of Mesalazine in tablet formulation. The data were statistically compared with those of a standard reference using Student's t-and F-test.