Combination of Gedi leaves (Abelmoschus Manihot L.) extract and arabica coffee (Coffea arabica L.) in making herbal coffee as an alternative therapy for diabetes mellitus

Gedi leaves (Abelmoschus manihot L.) contain many benefits, one of which is antidiabetic agents such as phenolic compound, flavonoid, and tannin. This research was conducted by adding Gedi leaves extract into Arabica coffee. The selection of coffee was based on the current trend, whereas societies commonly consume this product. The purpose of this research was to find out the best type of solvent used in extracting Gedi leaves to produce the best product (selected samples) from the formulation and compare between selected samples and synthetic medicines in their ability to lower blood glucose levels. This research consists of two-phase extraction of Gedi leaves with multiple maceration methods and in vitro test of inhibition of α-amylase (IC50), total phenolics and flavonoids test, and antioxidant activity test (IC50). Secondly, or the last, in vivo test of blood glucose levels using mice. The results of the α-amylase inhibition test (IC50), total phenolics, flavonoids, and antioxidant activity (IC50) showed that the initial 70% ethanol extract with a concentration of 50 ppm was the best sample (selected samples). When compared with synthetic medicines, the selected samples showed significantly different results, where that product had a better ability to lower blood glucose levels.