Ethanolic extract of Parkia speciosa Peel: Its effect on alkaline phosphatase and catalase level of wistar rats with “Jelantah” exposed

In Indonesia, it is common to re-use palm oil twice or more until the color of the oil is changed. This re-used palm oil is called “jelantah”. Palm oil that has been used for 10 hours or more will increase its fatty acid up to 0,5%, then make the oil unusable. Therefore, repeated use of palm oil may lead to organ damage, such as liver, kidney, and heart, due to fatty acid accumulation. Natural resources such as Parkia speciosa can reduce the risk of jelantah. P. speciosa can be easily found in Indonesia. Due to its large amount of phenol and flavonoid compounds, P. speciosa has antioxidant, antidiabetic, and antiangiogenic properties. The peel of P. speciosa can be used as a natural antioxidant to inhibit organ damage due to jelantah. Therefore, research about the effect of ethanolic extract of P. speciosa peel (EEPP) on alkaline phosphatase (ALP) and catalase (CAT) activity is necessary. In this research, Wistar rats were divided into four groups: group 1 with no treatment as the negative control, group 2 with 1 ml jelantah 118 meq/kg of body weight and 100 mg/L EEPP, group 3 with 1 ml jelantah 118 meq/kg of body weight and 200 mg/L EEPP, and group 4 as the positive control with 1 ml jelantah 118 meq/kg of body weight. The study showed that the ALP content in group 3 is significantly lower than the positive control (group 4). ALP content in group 2 is higher than group 3 but still lower than the positive control (group 4). The lowest ALP content showed in the negative control (group 1). This result indicated that the higher EEPP decreased the ALP content in Wistar rats with jelantah exposed. For CAT content, it showed that group 3 has higher CAT content than the positive control, significantly. The highest CAT content is group 1 (the negative control) and the lowest in group 4 (the positive control). CAT content in group 2 is significantly lower than group 3. It indicated that the higher EEPP increased the CAT level in Wistar rats with jelantah exposed.