Impact of wide-spectrum light in conjunction with laser irradiation on fibroblast proliferation

Objective: This research aimed at evaluating whether broad-spectrum light can support and accelerate cell proliferation in conjunction with laser therapy. Background Data: Broad-spectrum light can reduce the effect of laser light to some extent or completely. There are a few experiments that examine the gain or downside of integrating broad- spectrum light with laser irradiation. Methods: Fibroblasts were irradiated at various doses (J/cm2) using 635 and 808nm laser in the dark and light. Using MTT (3-(4,5-Dimethylthiazole-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) assays, changes in cell proliferation have been testing. Results: The findings show that low-level laser therapy (LLLT) has a beneficial effect on this cell type. The results revealed that dosages of 3.7, 5, 7.4, and 11 J/cm2 were adequate for fibroblast cells to undergo observable changes. The MTT assay shows that various dosages using 808 nanometers in the lit medium were more active than 635 nm in enhancing cell-reproduction and no as effective as 635 nm in the dark. Conclusion: Our findings show that cells respond optimally in the dark medium to wavelengths of 635 nm and in the light to wavelengths of 808 nm, preserving cell viability.