Partial purification of creatine kinase from serum of myocardial infection and study kinetic properties

This study was done to partial purification Creatine kinase from serum of myocardial infection patients and study enzyme kinetics under different conditions, a specimens were taken from the intensive care unit at Salah El-Din General Hospital for patients with myocardial infarction between 10/15/2020 and 29/1/2021 and the purification process by Sephadex G100 .the results of Kinetic studies showed that the optimal concentration of substrate was (30mmol), the Michael’s-Menten constant was (Km=5.88mmol), and the maximum velocity was (Vmax=1.66 IU/L), whereas the optimum temperature was (37°C) and the ideal pH was (7.4). Where the above practical results showed the activity of the enzyme at different pH, temperature and concentration of substrate concentration .