Evaluation of cytotoxicity of dental restorative nanometer hydroxyapatite composite resin in vitro by dentin barrier method

Objective: To determine the in vitro cytotoxicity of nano-hydroxyapatite composite resin by dentine barrier method and evaluate its biocompatibility, so as to provide theoretical basis for its application in oral clinic. Methods: A in vitro test device for the cytotoxicity of dentin barrier was made, and the cytotoxicity of nano hydroxyapatite composite resin, 3M Z350 composite resin, negative control material and positive control material were determined by this device. Results: The cell relative activity (%) of nano-hydroxyapatite composite resin and 3M Z350 composite resin determined by dentine barrier method were 92.5±3.63 and 89.1±1.63 respectively. Conclusion: Nano-hydroxyapatite composite resin is less cytotoxic than 3M Z350 composite resin, and can be safely applied in clinical practice.