The effect of detergent ingredients on stability of thermostable alkaline protease 50a in formulation of liquid stain remover

New detergents approach such as pre-dosed liquid and powder as pre-treatment to enhance cleaning efficiency has received great interest among consumer and is becoming a trend in laundry industry. Formulation of detergents is continuously developed to improve cleaning efficiency and to adapt and fulfill market demands. In this study, three liquid stain remover formulations containing thermostable alkaline protease 50a as additive were developed. The developed stain remover formulations were specifically designed to remove proteinaceous stains on fabrics. Azo-casein assay was performed to evaluate the stability of the thermostable alkaline protease 50a in each formulation. Wash performance analysis was also carried out to determine the stain removal efficiency of the developed formulations on selected fabrics namely satin, crepe and koshibo. The results obtained shows that the thermostable alkaline protease 50a lost more than 89% of its activity after one week of incubation in these three formulations. The developed stain remover formulation exhibited good stain removal efficiency (84.8-96.46%) in comparison to tap water (74.6-86.28%) and commercial stain remover (88.85-95.44%). The outcomes of this study could provide additional insights on the effect of detergent ingredients on the thermostable alkaline protease 50a activity.