Synthesis and toxicity test of 2’-hydroxy-5’-chloro-3,4-dymethoxychalcone

This study was aimed to synthesis the chalcone derivative and tested its toxicity. Synthesis of 2’-hydroxy-5’-chloro-3,4-dimethoxychalcone carried out through Claisen-Schmidt condensation at room temperature. The toxicity test prepared by Brine Shrimp Lethality Test (BSLT) to Artemia Salina L. Analysis retrosynthetic gives the 2-hydroxy-5-chloro acetophenone and 3,4-dimethoxy benzaldehyde as starting materials. The desire chalcone was confirmed by 1H and 13C NMR, also mass spectroscopy. The chalcone obtained in 83.27% and exhibited LC50 value in 974.99 µg/mL.