Identification of hemagglutinin protein from Streptococcus agalactiae pili in mice erythrocytes as a vaccine candidate

Streptococcus agalactiae is a gram-positive bacterium which has pili protein on its cell surface. It can cause pathogenic infections in the reproductive tract of women and newborns, resulting in premature births, meningitis, and pneumonia. Streptococcus agalactiae samples obtained from patients treated at Saiful Anwar Hospital Malang, East Java, Indonesia. Streptococcus agalactiae were cultured using biphasic brain heart infusion (BHI). The subjects were divided into 4 samples namely, the first pili cut sample, the second pili cut sample, the third pili cut sample and the whole Streptococcus agalactiae cells sample. Each group was tested for hemagglutination and SDS-PAGE electrophoresis. The analysis test result was seen based on the agglutination and the dominant color of the protein band using the EZ Gel-Biorad Gel Documentation System tool. The hemagglutination test showed that the second cut of Streptococcus agalactiae pili had the highest positive hemagglutination test at dilution of titer 64 (1/64). The SDS-PAGE electrophoresis test showed that the dominant bands were in the second pili cut sample at 35 and 53 kDa molecular weights. The pili Streptococcus agalactiae has high hemagglutination activity and dominant molecular weight, which can be used as vaccine candidates.