Optimization extracellular lipase activity from Moraxella sp SBE01 for hydrocarbons nanoremediation

The use of nano-scale materials such as bacterial lipase that catalyzes lipid breakdown has shown to be potentially effective for hydrocarbons nanoremediation. The activity of the lipase on degradation of those pollutants is highly depends on several factors, such as temperature, pH and ion compounds. The new isolate of Moraxella sp SBE01 was investigated in terms of lipase production, optimal conditions (temperatures, pHs), and also observed regarding the effect of ions on its enzyme activity. The range of temperatures and pH values examined in this research were 20°C-80°C and pH 5.0-12, respectively. The experiment revealed that crude lipase of the strain could be harvested highly at 20 hours incubation. Partially purified lipase using ammonium sulfate precipitation followed by dialysis showed its optimum activity was observed at a temperature of 50°C and pH 7 with specific activities were 9.05 and 9.07 U/mg protein, respectively. Ion compounds such as CaO and MgO at 0.1 mM increased that activity of 12.97 and 11.93 U/mg protein respectively. However, Fe2O3 and MnO2 at 0.1 mM decreased that activity of 3.09 and 4.62 U/mg protein, respectively. These particular characters of the partially purified lipase Moraxella sp SBE01 make it promising as a biocatalyst for nanoremediation.