Design of experiments based factorial design and usability of response surface methodology for saponin extraction from tribulus territris

According to the ancient terms, herbs are the major source in the treatment of various acute diseases. Various herbs are used as major sources, in which Tribulus is one of rich sources of high medicinal values. The saponin content in the tribulus herb is at high rate compared to other Phyto-chemicals. The high rated saponin content extracted from the tribulus in turn responsible for the treatment of various diseases. So, the ethanol extraction of saponin is performed through soxhlet apparatus. In the present work, the extraction procedure is performed, optimization of the parameters like different dosage, time and temperature etc are analyzed by Response surface methodology. The response surface is resulted with highest removal i.e 88.45% with a desirability of 0.774 when parameters are optimized at S/L Ratio of 1.25, Time 234.47 min and Temp 75°C is maintained.