Virulence gene of Pseudomonas aeruginosa with nanoparticle

Pseudomonas aeruginosa from specimens of Diabetic Foot patients carries a high risk of misidentification. That leads to a non-specific treatment by antibiotic, so that present study was target virulence gene as specific gene Cup A gene, for the accurate and rapid identification of P. aeruginosa from Diabetic Foot Patients using sequencing for specific virulence genes Cup A gene, that gene facilitate the infection as adhesion gene. This technique was evaluated against 20 clinical isolates that were suspected as P. aeruginosa. The results were compared with those obtained using as a control of P. aeruginosa isolates from clinical samples. This reliable technique may offer a rapid tool that would help clinicians to initiate an appropriate treatment earlier. Further investigations are needed to assess the clinical benefit of this novel strategy as compared to antibiotic using. Nanoparticles (NPs) are increasingly used to target bacteria as an alternative to antibiotics. Nanotechnology may be particularly advantageous in treating bacterial infections. The antibacterial mechanisms of NPs against bacteria and the factors that are involved for comparison. Evaluation the result of nanoparticles on Bacteria by studying the genetic variation in bacterial genome before and after treatment was the aim of present study. Present results showed genetic variation for bacterial adhesion gene Cup A, the deletion mutation was repeated 5 times at the cytosine base, resulting in a breakthrough from Frame shift. Deletion Cytosine more than any other nitrogen base needs more investigation.