The role of sucrose as extracellular cryoprotectant in maintaining the Garut rams’ frozen semen quality

The main objective of this research was to use a Tris extender which was supplemented with some sucrose concentration as to acquire the best quality of Garuts rams frozen semen. The result showed that the percentages of progressive motile sperm for additional sucrose of 0,4 g / 100 ml extender (49,0...

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Hauptverfasser:	Herdis, Surachman, M., Darmawan, I. W. A., Afifah
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Sprache:	eng
Schlagworte:	Artificial insemination Cryogenic engineering Cryoprotectants Reliability analysis Semen Sperm Sucrose
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creator	Herdis Surachman, M. Darmawan, I. W. A. Afifah
description	The main objective of this research was to use a Tris extender which was supplemented with some sucrose concentration as to acquire the best quality of Garuts rams frozen semen. The result showed that the percentages of progressive motile sperm for additional sucrose of 0,4 g / 100 ml extender (49,00 ± 5,48%) was significantly higher (P < 0,05) than control (42,00 ± 2,74%) but was not significantly different (P > 0,05) from additional sucrose of 0,2 g / 100 ml extender (46,00 ± 4,18%). The percentages of sperm life for additional sucrose of 0,4 g / 100 ml extender (66,80 ± 5,81%) was significantly higher (P < 0,05) than control (52,67 ± 1,51%) but was not significantly different (P > 0,05) from additional sucrose of 0,2 g / 100 ml extender (62,75 ± 4,79 %). The percentages of plasma membrane intact of sperm for additional sucrose of 0,2 g / 100 ml extender (62,33 ± 6,51%) was significantly higher than (P < 0,05) the control (49,40 ± 2,19%). In conclusion, the additional of sucrose into the semen extenders could improve the quality of the Garut ram’s frozen semen. The addition of sucrose 0,2 g/100 ml extender is optimal dose to improve the quality of frozen semen of Garut rams.
doi_str_mv	10.1063/1.5115757
format	Conference Proceeding
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W. A. ; Afifah</creator><contributor>Romaidi ; Wahyudi, Didik ; Kikuchi, Akira ; Daryono, Retno Novvitasari Hery ; Yusnawan, Eriyanto</contributor><creatorcontrib>Herdis ; Surachman, M. ; Darmawan, I. W. A. ; Afifah ; Romaidi ; Wahyudi, Didik ; Kikuchi, Akira ; Daryono, Retno Novvitasari Hery ; Yusnawan, Eriyanto</creatorcontrib><description>The main objective of this research was to use a Tris extender which was supplemented with some sucrose concentration as to acquire the best quality of Garuts rams frozen semen. The result showed that the percentages of progressive motile sperm for additional sucrose of 0,4 g / 100 ml extender (49,00 ± 5,48%) was significantly higher (P < 0,05) than control (42,00 ± 2,74%) but was not significantly different (P > 0,05) from additional sucrose of 0,2 g / 100 ml extender (46,00 ± 4,18%). The percentages of sperm life for additional sucrose of 0,4 g / 100 ml extender (66,80 ± 5,81%) was significantly higher (P < 0,05) than control (52,67 ± 1,51%) but was not significantly different (P > 0,05) from additional sucrose of 0,2 g / 100 ml extender (62,75 ± 4,79 %). The percentages of plasma membrane intact of sperm for additional sucrose of 0,2 g / 100 ml extender (62,33 ± 6,51%) was significantly higher than (P < 0,05) the control (49,40 ± 2,19%). In conclusion, the additional of sucrose into the semen extenders could improve the quality of the Garut ram’s frozen semen. The addition of sucrose 0,2 g/100 ml extender is optimal dose to improve the quality of frozen semen of Garut rams.</description><identifier>ISSN: 0094-243X</identifier><identifier>EISSN: 1551-7616</identifier><identifier>DOI: 10.1063/1.5115757</identifier><identifier>CODEN: APCPCS</identifier><language>eng</language><publisher>Melville: American Institute of Physics</publisher><subject>Artificial insemination ; Cryogenic engineering ; Cryoprotectants ; Reliability analysis ; Semen ; Sperm ; Sucrose</subject><ispartof>AIP conference proceedings, 2019, Vol.2120 (1)</ispartof><rights>Author(s)</rights><rights>2019 Author(s). 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A.</creatorcontrib><creatorcontrib>Afifah</creatorcontrib><title>The role of sucrose as extracellular cryoprotectant in maintaining the Garut rams’ frozen semen quality</title><title>AIP conference proceedings</title><description>The main objective of this research was to use a Tris extender which was supplemented with some sucrose concentration as to acquire the best quality of Garuts rams frozen semen. The result showed that the percentages of progressive motile sperm for additional sucrose of 0,4 g / 100 ml extender (49,00 ± 5,48%) was significantly higher (P < 0,05) than control (42,00 ± 2,74%) but was not significantly different (P > 0,05) from additional sucrose of 0,2 g / 100 ml extender (46,00 ± 4,18%). The percentages of sperm life for additional sucrose of 0,4 g / 100 ml extender (66,80 ± 5,81%) was significantly higher (P < 0,05) than control (52,67 ± 1,51%) but was not significantly different (P > 0,05) from additional sucrose of 0,2 g / 100 ml extender (62,75 ± 4,79 %). The percentages of plasma membrane intact of sperm for additional sucrose of 0,2 g / 100 ml extender (62,33 ± 6,51%) was significantly higher than (P < 0,05) the control (49,40 ± 2,19%). In conclusion, the additional of sucrose into the semen extenders could improve the quality of the Garut ram’s frozen semen. The addition of sucrose 0,2 g/100 ml extender is optimal dose to improve the quality of frozen semen of Garut rams.</description><subject>Artificial insemination</subject><subject>Cryogenic engineering</subject><subject>Cryoprotectants</subject><subject>Reliability analysis</subject><subject>Semen</subject><subject>Sperm</subject><subject>Sucrose</subject><issn>0094-243X</issn><issn>1551-7616</issn><fulltext>true</fulltext><rsrctype>conference_proceeding</rsrctype><creationdate>2019</creationdate><recordtype>conference_proceeding</recordtype><recordid>eNp9kM1KAzEUhYMoWKsL3yDgTpia_8wspWgVCm4quAuZTEZTZibTJCPWVV_D1_NJHGnBnYt77ua751wOAJcYzTAS9AbPOMZccnkEJphznEmBxTGYIFSwjDD6cgrOYlwjRAop8wlwqzcLg28s9DWMgwk-WqgjtB8paGObZmh0gCZsfR98sibpLkHXwVa7Lo3juleYRouFDkOCQbfxe_cF6-A_bQejbUfdDLpxaXsOTmrdRHtx2FPwfH-3mj9ky6fF4_x2mfUkz1PGhLBVXuHc5IaLuiAlr0pbEsFtWTODjSlLIilGUuqiqCgnVBMmasE4sboidAqu9r7jw5vBxqTWfgjdGKkI4ZghTpkYqes9FY1LOjnfqT64VoetevdBYXWoUfVV_R-Mkfrt_e-A_gAlNHdj</recordid><startdate>20190703</startdate><enddate>20190703</enddate><creator>Herdis</creator><creator>Surachman, M.</creator><creator>Darmawan, I. 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A. ; Afifah</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p288t-466ed8d18c8c56f92b5dbeb265ebf4c1ccbb2731077a99d3523a246f6452ead23</frbrgroupid><rsrctype>conference_proceedings</rsrctype><prefilter>conference_proceedings</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Artificial insemination</topic><topic>Cryogenic engineering</topic><topic>Cryoprotectants</topic><topic>Reliability analysis</topic><topic>Semen</topic><topic>Sperm</topic><topic>Sucrose</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Herdis</creatorcontrib><creatorcontrib>Surachman, M.</creatorcontrib><creatorcontrib>Darmawan, I. W. A.</creatorcontrib><creatorcontrib>Afifah</creatorcontrib><collection>Technology Research Database</collection><collection>Aerospace Database</collection><collection>Advanced Technologies Database with Aerospace</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Herdis</au><au>Surachman, M.</au><au>Darmawan, I. W. A.</au><au>Afifah</au><au>Romaidi</au><au>Wahyudi, Didik</au><au>Kikuchi, Akira</au><au>Daryono, Retno Novvitasari Hery</au><au>Yusnawan, Eriyanto</au><format>book</format><genre>proceeding</genre><ristype>CONF</ristype><atitle>The role of sucrose as extracellular cryoprotectant in maintaining the Garut rams’ frozen semen quality</atitle><btitle>AIP conference proceedings</btitle><date>2019-07-03</date><risdate>2019</risdate><volume>2120</volume><issue>1</issue><issn>0094-243X</issn><eissn>1551-7616</eissn><coden>APCPCS</coden><abstract>The main objective of this research was to use a Tris extender which was supplemented with some sucrose concentration as to acquire the best quality of Garuts rams frozen semen. The result showed that the percentages of progressive motile sperm for additional sucrose of 0,4 g / 100 ml extender (49,00 ± 5,48%) was significantly higher (P < 0,05) than control (42,00 ± 2,74%) but was not significantly different (P > 0,05) from additional sucrose of 0,2 g / 100 ml extender (46,00 ± 4,18%). The percentages of sperm life for additional sucrose of 0,4 g / 100 ml extender (66,80 ± 5,81%) was significantly higher (P < 0,05) than control (52,67 ± 1,51%) but was not significantly different (P > 0,05) from additional sucrose of 0,2 g / 100 ml extender (62,75 ± 4,79 %). The percentages of plasma membrane intact of sperm for additional sucrose of 0,2 g / 100 ml extender (62,33 ± 6,51%) was significantly higher than (P < 0,05) the control (49,40 ± 2,19%). In conclusion, the additional of sucrose into the semen extenders could improve the quality of the Garut ram’s frozen semen. The addition of sucrose 0,2 g/100 ml extender is optimal dose to improve the quality of frozen semen of Garut rams.</abstract><cop>Melville</cop><pub>American Institute of Physics</pub><doi>10.1063/1.5115757</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
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source	AIP Journals Complete
subjects	Artificial insemination Cryogenic engineering Cryoprotectants Reliability analysis Semen Sperm Sucrose
title	The role of sucrose as extracellular cryoprotectant in maintaining the Garut rams’ frozen semen quality
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