Verifying the competition between haloperidol and biperiden in serum albumin through a model based on spectrofluorimetry

The aim of this work was to apply mathematical-computational modeling to study the interactions of haloperidol (HLP) and biperiden (BPD) with human (HSA) and bovine (BSA) serum albumin in order to verify the competition of these drugs for binding sites in HSA, using intrinsic tryptophan fluorescence quenching data. The association constants estimated for HPD-HSA was 2.17(±0.05) × 107 M−1, BPD-HSA was 2.01(±0.03) × 108 M−1 at 37 °C. Results have shown that drugs do not compete for the same binding sites in albumin.