Cell micro-irradiation with MeV protons counted by an ultra-thin diamond membrane

We report the development of thin single crystal diamond membranes suitable for dose control in targeted cell irradiation experiments with a proton microbeam. A specific design was achieved to deliver single protons with a hit detection efficiency approaching 100%. The membranes have thicknesses bet...

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Veröffentlicht in:	Applied physics letters 2017-12, Vol.111 (24)
Hauptverfasser:	Barberet, Philippe, Pomorski, Michal, Muggiolu, Giovanna, Torfeh, Eva, Claverie, Gérard, Huss, Cédric, Saada, Samuel, Devès, Guillaume, Simon, Marina, Seznec, Hervé
Format:	Artikel
Sprache:	eng
Schlagworte:	Applied physics Computer Science Computer simulation Deoxyribonucleic acid Diamonds DNA Engineering Sciences Fluorescence Instrumentation and Detectors Irradiation Membranes Micro and nanotechnologies Microbeams Microelectronics Modeling and Simulation Monte Carlo simulation Nuclear Experiment Physics Proteins Proton beams Single crystals
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Zusammenfassung:	We report the development of thin single crystal diamond membranes suitable for dose control in targeted cell irradiation experiments with a proton microbeam. A specific design was achieved to deliver single protons with a hit detection efficiency approaching 100%. The membranes have thicknesses between 1.8 and 3 μm and are used as vacuum windows on the microbeam line. The impact of these transmission detectors on the microbeam spot size is estimated by Monte-Carlo simulations, indicating that a beam lateral resolution below 2 μm is achieved. This is confirmed by experiments showing the accumulation online of X-ray Repair Cross-Complementing protein 1 (XRCC1)-Green Fluorescent Protein (GFP) at DNA damaged sites in living cells.
ISSN:	0003-6951 1077-3118
DOI:	10.1063/1.5009713