Buckwheat seed quality and pathogenicity of Fusarium spp. in plants
Buckwheat is a species with great economic and production potential, which has gained increasing importance. This study aimed to determine the physical, physiological, and sanitary quality of samples from seed lots, and to evaluate the pathogenicity of Fusarium spp. in buckwheat plants. Physical and...
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Veröffentlicht in: | Journal of seed science 2022, Vol.44 |
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Sprache: | eng |
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Zusammenfassung: | Buckwheat is a species with great economic and production potential, which has gained increasing importance. This study aimed to determine the physical, physiological, and sanitary quality of samples from seed lots, and to evaluate the pathogenicity of Fusarium spp. in buckwheat plants. Physical and physiological quality was evaluated by the thousand-seed weight, moisture content, germination test (5th and 7th days), seedling length, and seedling dry mass, while sanitary quality was determined by the health test on filter paper, with the seeds subjected to asepsis and without asepsis. Isolates of Fusarium spp. were obtained from symptomatic seedlings in the paper roll germination test. To identify and characterize fungal isolates, morphological and molecular approaches were used. Pathogenicity was determined on healthy plants in a controlled environment. The lots showed high physiological quality in the germination evaluation (5th and 7th days). There was a high incidence of Fusarium spp. in all lots, which can be reduced with seed asepsis. The isolates were identified as Fusarium incarnatum-equiseti species complex, and were pathogenic to buckwheat plants.
Resumo: O trigo mourisco é uma espécie com grande potencial econômico e de produção, a qual tem ganhado cada vez mais importância. O objetivo deste estudo foi determinar a qualidade física, fisiológica e sanitária de amostras provenientes de lotes de sementes, e avaliar a patogenicidade de Fusarium spp. em plantas de trigo mourisco. A qualidade física e fisiológica foi avaliada pelo peso de mil sementes, grau de umidade, teste de germinação (5° e 7° dias), comprimento de plântula e massa seca de plântula, enquanto que a qualidade sanitária foi determinada pelo teste de sanidade em papel filtro, com as sementes submetidas à assepsia e sem assepsia. Os isolados de Fusarium spp. foram obtidos de plântulas sintomáticas no teste de germinação em rolo de papel. Para a identificação e caracterização dos isolados fúngicos foram utilizadas abordagens morfológicas e moleculares. A patogenicidade foi realizada em plantas sadias em ambiente controlado. Os lotes apresentaram alta qualidade fisiológica na avaliação de germinação (5° e 7° dias). Houve alta incidência de Fusarium spp. em todos os lotes, a qual pode ser diminuída com a assepsia das sementes. Os isolados foram identificados como complexo de espécies de Fusarium incarnatum-equiseti, e foram patogênicos às plantas de trigo mourisco. |
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ISSN: | 2317-1537 2317-1545 2317-1545 |
DOI: | 10.1590/2317-1545v44256994 |