Pedigree testing for the SCS425 Luiza apple cultivar

According to literature, the apple cultivar SCS425 Luiza is a hybrid of Imperatriz♀ and Cripps Pink♂; however, molecular analysis of gametophytic selfincompatibility alleles showed the presence of an unexpected allele in SCS425 Luiza. This raised doubts about the fidelity of the pedigree. Thus, this study aimed to investigate the real genealogy of the SCS425 Luiza cultivar via fingerprint analysis. A total of 19 pairs of SSR primers covering 12 of the 17 chromosomes were used. ‘Imperatriz’ was tested as the female parent, and either ‘Cripps Pink’ or ‘Baronesa’ was tested as the presumed male parents. The results excluded ‘Cripps Pink’ as the male parent because most of the markers showed alleles of exclusion with Likelihood ratio (LR) value equal to zero. In contrast, when Baronesa was tested as the male parent, all alleles followed the expected segregation with very high LR values (>10.000). Therefore, it is concluded that the correct genealogy of the SCS425 Luiza cultivar is Imperatriz♀ and Baronesa♂.