Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples

Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples

The aim of this study was to compare the detection of Ehrlichia canis morulae and DNA by nPCR in whole blood and spleen aspiration. The sample included 40 dogs showing thrombocytopenia associated to clinical signs suggestive of canine ehrlichiosis. Morulae detection showed that in 35 of the dogs stu...

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Veröffentlicht in:Revista brasileira de parasitologia veterinaria 2010-06, Vol.19 (2), p.98-102
Hauptverfasser: Faria, J. L. M., Dagnone, A. S., Munhoz, T. D., João, C. F., Pereira, W. A. B., Machado, R. Z., Tinucci-Costa, M.
Format: Artikel
Sprache:eng
Schlagworte:
PARASITOLOGY
VETERINARY SCIENCES
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Zusammenfassung:The aim of this study was to compare the detection of Ehrlichia canis morulae and DNA by nPCR in whole blood and spleen aspiration. The sample included 40 dogs showing thrombocytopenia associated to clinical signs suggestive of canine ehrlichiosis. Morulae detection showed that in 35 of the dogs studied, 17 had morulae in spleen tissue, and two in buffy coat smears. E. canis DNA was detected in 29/40 blood samples. We verified that morulae detection is more efficient in cytological preparations from spleen aspiration. On the other hand, nPCR on spleen and blood samples were equally efficient for disease diagnosis.
ISSN:1984-2961
1984-2961
DOI:10.4322/rbpv.01902006