Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex

We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169 C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very usefu...

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Veröffentlicht in:	Brazilian journal of microbiology 2014-07, Vol.45 (3), p.841-843
Hauptverfasser:	Spositto, F L E, Campanerut, P A Z, Ghiraldi, L D, Leite, C Q F, Hirata, M H, Hirata, R D C, Siqueira, V L D, Cardoso, R Fressatti
Format:	Artikel
Sprache:	eng
Schlagworte:	Amidohydrolases - genetics Bacteria Bacteriological Techniques - methods Cattle DNA Transposable Elements DNA, Bacterial - genetics MICROBIOLOGY Molecular Diagnostic Techniques - methods Multiplex Polymerase Chain Reaction - methods Mycobacterium bovis - classification Mycobacterium bovis - genetics Mycobacterium bovis - isolation & purification Mycobacterium tuberculosis - classification Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Pathogens Short Communication Tuberculosis Tuberculosis - diagnosis Tuberculosis - microbiology
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Beschreibung
Zusammenfassung:	We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169 C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.
ISSN:	1517-8382 1678-4405 1678-4405
DOI:	10.1590/S1517-83822014000300012