Measuring of Mycobacterium tuberculosis growth. A correlation of the optical measurements with colony forming units

The quantification of colony forming units (cfu), turbidity, and optical density at 600 nm (OD600) measurements were used to evaluate Mycobacterium tuberculosis growth. Turbidity and OD600 measurements displayed similar growth curves, while cfu quantification showed a continuous growth curve. We det...

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Veröffentlicht in:Brazilian journal of microbiology 2013-01, Vol.44 (1), p.287-290
Hauptverfasser: Peñuelas-Urquides, Katia, Villarreal-Treviño, Licet, Silva-Ramírez, Beatriz, Rivadeneyra-Espinoza, Liliana, Said-Fernández, Salvador, de León, Mario Bermúdez
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Sprache:eng
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Zusammenfassung:The quantification of colony forming units (cfu), turbidity, and optical density at 600 nm (OD600) measurements were used to evaluate Mycobacterium tuberculosis growth. Turbidity and OD600 measurements displayed similar growth curves, while cfu quantification showed a continuous growth curve. We determined the cfu equivalents to McFarland and OD600 units.
ISSN:1517-8382
1678-4405
1678-4405
DOI:10.1590/S1517-83822013000100042