Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA

Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA

This study aimed to evaluate the use of the FTA elute card(TM) impregnated with cervicovaginal sample directly in the PCR amplification for detection of HPV-DNA. The results were compared to a reference technique. This method was more efficient than the protocol indicated by the manufacturer, identi...

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Veröffentlicht in:Brazilian journal of microbiology 2012-01, Vol.43 (1), p.389-392
Hauptverfasser: Santos, Carla R, Franciscatto, Laura G, Barcellos, Regina B, Almeida, Sabrina E M, Rossetti, Maria Lucia R
Format: Artikel
Sprache:eng
Schlagworte:
Cervical cancer
Human papillomavirus
MICROBIOLOGY
Polymerase chain reaction
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Zusammenfassung:This study aimed to evaluate the use of the FTA elute card(TM) impregnated with cervicovaginal sample directly in the PCR amplification for detection of HPV-DNA. The results were compared to a reference technique. This method was more efficient than the protocol indicated by the manufacturer, identifying 91.7% against 54.2% of the positive samples.
ISSN:1517-8382
1678-4405
1678-4405
DOI:10.1590/S1517-83822012000100047