Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil

Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phyl...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Brazilian journal of microbiology 2011-10, Vol.42 (4), p.1440-1444
Hauptverfasser:	Vaucher, Rodrigo de Almeida, Dezen, Diogenes, Simonetti, Amauri Braga, Spilki, Fernando Rosado, Roehe, Paulo Michel
Format:	Artikel
Sprache:	eng
Schlagworte:	Agricultural biotechnology Cattle Influenza MICROBIOLOGY Monoclonal antibodies Phylogenetics
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3. [PUBLICATION ABSTRACT]
ISSN:	1517-8382 1678-4405 1678-4405
DOI:	10.1590/S1517-83822011000400029