Rapid polyvalent screening for largescale environmental Spiroplasma surveys

Surface serology is an important determinant in Spiroplasma systematics. Reciprocal antigen/antibody reactions between spiroplasmas and individual antisera delineate the 38 described groups and species. However, reciprocal serology is impractical for largescale studies. This report describes a succe...

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Veröffentlicht in:Brazilian journal of microbiology 2009-09, Vol.40 (3), p.663-669
Hauptverfasser: French, Frank E.(Georgia Southern University Institute of Arthropodology and Parasitology Department of Biology), Whitcomb, Robert F.(U. S. Department of Agriculture Vegetable Laboratory), Williamson, David L.(State University of New York Department of Anatomical Sciences), Regassa, Laura B.(Georgia Southern University Institute of Arthropodology and Parasitology Department of Biology)
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Sprache:eng
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Zusammenfassung:Surface serology is an important determinant in Spiroplasma systematics. Reciprocal antigen/antibody reactions between spiroplasmas and individual antisera delineate the 38 described groups and species. However, reciprocal serology is impractical for largescale studies. This report describes a successful, streamlined polyvalent screening approach used to examine isolates from an environmental survey. A sorologia de superfície é um determinante importante na sistemática de Spiroplasma. Reações antígeno-anticorpo entre spiroplasmas e antisoro individuais delineiam os 38 grupos e espécies descritos. No entanto, reações sorológicas são impraticáveis em estudos em larga-escala. Esse relato descreve uma metodologia de triagem bem sucedida a ser empregada no exame de isolados em levantamentos ambientais.
ISSN:1517-8382
1678-4405
1678-4405
DOI:10.1590/S1517-83822009000300031